email: info@sdr.com.au Sydney, Australia Tel: +61-2-9882-2882

Digital Cameras

for Fluorescence and Live Cell Imaging

We supply a range of cooled CCD and advanced CMOS cameras, especially suitable for fluorescence microscopy and live cell imaging, from Hamamatsu. All the models mentioned here are compatible with HCImage imaging software.

Hamamatsu offers a full lineup of quality models covering such diverse needs as Ca++ and ratio imaging, FRET, TIRF, live cell and timelapse imaging, FISH, confocal microscopy and physical science applications.

This page shows just some of our most popular camera models - please feel free to contact us to discuss how your particular needs can be met from your budget, or for advice on choosing a camera.

Cameras can be a little complicated! For more information:

The Living Image - a new Life Science Imaging site from Hamamatsu

New Camera Summary & Comparison Brochure (6.1MB)

 

Most Popular Cameras

ORCA-Flash4.0 New - A revolutionary sCMOS camera: 4 megapixels, 100 frame/sec at full resolution, high QE & low noise
ORCA-Flash2.8 Advanced CMOS device 1920 x 1440 pixels, 45 frames/s, low noise
ImageEM On-chip electron multipler gain, for very low light; 512x512 or 1024x1024 models
ORCA-R2 Deep cooled CCD, 1344x1024 pixels, with versatile settings for high speed or long exposures
ORCDA-D2 Dual CCD chips with inserted optical block for simultaneous dual-wavelength imaging
ORCA-03G, -05G Affordable cooled (-03G) and uncooled (-05G) models for brightfield and fluorescence
C9300-221 High frame rate CCD camera with high IR sensitivity
ORCA-3CCD Three-chip colour camera, with 3 cooled 1344x1024 pixel CCD chips (R, G and B)

 

ORCA-Flash4.0 - Changing the Game

Hamamatsu's brilliantly designed ORCA-Flash4.0 is truly a game changer in the world of scientific imaging. Built on a revolutionary new Gen II sCMOS detector, the ORCA-Flash4.0 is the first sCMOS camera that challenges the performance of all CCD, EM-CCD, and Gen-I sCMOS cameras.

With its combination of low noise and high quantum efficiency, the ORCA-Flash4.0 delivers unprecedented sensitivity as well as high dynamic range, blazing fast speeds, large field of view, and excellent resolution - all at once. Now one camera covers a wide range of imaging needs - including localization microscopy, TIRF microscopy, live cell GFP, high-speed calcium ion imaging, FRET, real-time confocal microscopy, and many more.

The new V2 camera (shipping 03/13) adds a slow readout mode with even lower noise.

  • Exceptional sensitivity: over 70% quantum efficiency at 600 nm
  • Low noise: 1.3 electrons at 100 frames per second (0.9 electrons with slow readout)
  • High resolution: 4.0 megapixels with 6.5 x 6.5 µm pixel size
  • Offers a field of view 2.5 times larger than a standard EM-CCD.
  • High speed: 100 frames per second at full resolution
  • Even higher speeds with a smaller region of interest, e.g. 400 frames/sec at 2048 x 512
Hamamatsu ORCA-Flash-4.0

Hamamatsu's Pages on the ORCA-FLASH-4.0

Changing The Game - A White Paper (1.4MB)

ORCA-Flash4.0 Datasheet (919kB)

 

ORCA-Flash2.8 - the new Scientific CMOS sensor

The ORCA-Flash2.8 is based on the new FL-280 scientific image sensor. This is an advanced CMOS device giving high resolution, high readout speed and low noise simultaneously.

The ORCA-Flash2.8 provides 2.8 megapixel resolution at 45 frames/s (and up to 1273 frames/s by sub-array readout) while achieving lower readout noise than a cooled CCD camera.

Applications include Ca++ and ratio imaging, live cell imaging with GFP, FRET, TIRF, FISH, confocal microscopy and physical science applications.

  • Fast readout - 45 frames/s at full resolution of 1920 x 1440
  • Up to 1273 frames/s with sub array readout
  • Low dark current with internal cooling to 5 °C
  • 3 electrons r.m.s. noise performance
  • On-chip gain control 1x to 8x (in 256 steps)
  • Exposure range 20 μs to 10 s
  • Dynamic range 4500:1, 12-bit readout
Hamamatsu ORCA-Flash-2.8

ORCA-Flash2.8 Datasheet (271kB)

ORCA-Flash2.8 Technical Notes (413kB)

 

C9100 Electron Multiplier Cameras

The C9100 series gathers together all the features you would expect in a top-end camera: high gain, high signal to noise ratio, high resolution and speed, thanks to a new generation EM-CCD sensor.

With on-chip multiplication technology of the EM-CCD sensor the signal is drastically boosted while readout noise value is kept less than 1 electron r.m.s. at high gain mode. The gain factor of the C9100 family is up to 2000, making really low light applications a breeze.

The NEW ImagEM-X2 (C9100-23B) adds additional speed without a noise sacrifice: 70 frames/s at full resolution (512 x 512) and a blistering 1076 frames/s using an ROI of 16 x 16 pixels (using 4x binning). This masterfully redesigned camera delivers maximum speed and precision performance.

Hamamatsu C9100

C9100-13/14 EM-CCD Camera Datasheet (702kB)
C9100-23B EM-CCD Camera Datasheet (300kB)

C9100-14

  • Resolution 1024 x 1024; sub-array readout available
  • Backthinned frame transfer CCD
  • Quantum efficiency > 90% at peak wavelength
  • Electron multiplier gain max. 1200 times
  • Analog gain 0.5 to 5 times
  • Both water- and air-cooling provided
  • Cooled to -70 ºC (water) or -75 ºC (air)
  • Frame rate 9.5 Hz (60 Hz with 8x8 binning, higher for sub-arrays)
  • 16-bit readout
  • Dynamic range ~400000:1 (at max EM gain)

C9100-13

  • Resolution 512 x 512; sub-array readout available
  • Backthinned frame transfer CCD
  • Quantum efficiency > 90% at peak wavelength
  • Electron multiplier gain max. 1200 times
  • Analog gain 0.5 to 5 times
  • Both water- and air-cooling provided
  • Cooled to -80 ºC (water) or -65 ºC (air)
  • Frame rate 32 Hz (177 Hz with 8x8 binning, higher for sub-arrays)
  • 16-bit readout
  • Dynamic range ~370000:1 (at max EM gain)

C9100-23B

  • Resolution 512 x 512; sub-array readout available
  • Backthinned frame transfer CCD
  • Quantum efficiency > 90% at peak wavelength
  • Electron multiplier gain max. 1200 times
  • Both water- and air-cooling provided
  • Cooled to -80 ºC (water) or -65 ºC (air)
  • Frame rate 70 Hz at full resolution, up to 1076 Hz with binning and sub-arrays
  • 16-bit readout
  • Dynamic range ~370000:1 (at max EM gain)
Hamamatsu ORCA-R2

Orca-R2 Datasheet (450kB)

ORCA-R2 for Maximum Versatility

The ORCA-R2 is a high resolution digital camera designed for both high speed and high sensitivity. It has a choice of very low noise or very fast readout, to suit the application. It also has a choice of 12-bit and 16-bits modes under software control. Ideal for fluorescence microscopy, live cell imaging and a range of techniques such as FISH and ratio imaging, the Orca-R2 replaces the very popular Orca-AG.

  • Progressive scan interline ER-150 CCD - shutterless
  • Extended range: high sensitivity in VIS-NIR region
  • IEEE 1394 (firewire) interface
  • Resolution 1344 x 1024; sub-array readout available
  • Cooled to -35 °C (air cooling) or -40 °C (water cooling)
  • Exposure time 10 μs to 4200 s
  • Frame rate 8.5 Hz (low-noise readout) or 16.2 Hz (fast readout), full frame
  • Frame rates up to 115 Hz with 8x8 binning
  • 12-bit readout; Dynamic range ~3000:1

 

Orca-D2 Dual wavelength camera

The Orca-D2 is a new and unique dual-chip CCD camera, allowing two images at different wavelengths to be captured simultaneously and with a wide field of view. User-interchangeable optical blocks are used to set the wavelength ranges for each CCD. During image capture, the camera automatically corrects focus, alignment and colour shifting to produce high quality images. The camera is ideal for ratio imaging, FRET, and colocalisation and FISH applications.

  • Resolution 1280 x 960 (each CCD)
  • Progressive scan interline ER-150 CCD - shutterless
  • IEEE 1394 (firewire) interface
  • Cooled to -10 °C
  • Exposure time 117 μs to 60 s
  • Frame rate 11 Hz full frame, 33.5 Hz with 8x8 binning
  • 12-bit readout; Dynamic range ~2250:1
  • Optical bocks available for CFP/YFP; FITC/TRITC; Cy3/Cy5
Hamamatsu Orca-D2

Orca-D2 Camera Datasheet (480kB)

 

Hamamatsu ORCA-03G

Orca-03G/05G Datasheet (247kB)

ORCA-03G and ORCA-05G

The ORCA-03G and ORCA-05G are affordable CCD cameras suitable for routine fluorescence microscopy, GFP analysis, FISH, histology, pathology and a range of physical science applications. They use the popular IEEE1394 (Firewire) interface for simple interfacing to your computer, and are supplied with HCImage-Live - a complimentary image acquisition program. The -03G is moderately cooled, and the -05G is uncooled.

  • Progressive scan interline ER-150 CCD - shutterless
  • IEEE 1394 (firewire) interface
  • Resolution 1344 x 1024; sub-array readout available
  • Cooled to -10 °C (Orca-03G) or uncooled (Orca-05G)
  • Exposure time 10 μs to 10 s (Orca-03G); 10 μs to 1 s (Orca-05G)
  • Frame rate 8.9 Hz full frame; 43 Hz with 8x8 binning
  • 12-bit readout; Dynamic range ~2140:1 (Orca-03G) or ~1500:1 (Orca-05G)

 

C9300-221 High Frame Rate camera

The C9300-221 is a VGA format high speed CCD camera using progressive interline transfer with no mechanical shutter. It has a 33 μs electronic shutter capability, and a full frame readout of 82 of 150 frames/second. The high NIR sensitivity makes it an excellent choice for NIR-DIC specimens, or for stop action imaging of fast moving fluorescent specimens.

  • Resolution 640 x 480 pixels
  • Progressive scan interline tranfer CCD
  • Wide range sensitivity from blue to NIR
  • Cooled to 0 to 5 °C
  • Exposure time 33 μs to 10 s
  • Frame rate 82 Hz at 1x1 binning, up to 694 Hz 8x8 binning with dual readout
  • 12-bit readout; Dynamic range ~1000:1
Hamamatsu C9330-221
C9300-221 Camera Datasheet (175kB)

 

ORCA-3CCD 3-chip Colour Camera

ORCA-3CCD 3-chip Colour CCD

ORCA-3CCD Camera Datasheet (47kB)

The Orca-3CCD color camera provides very high fidelity 36 bit real color images in a single exposure, but with independent exposure times for each of the Red, Green, and Blue CCDs. Shutter free interline transfer design and cooling of the CCDs to 0 °C makes the camera useful for both brightfield color applications as well as high dynamic range multiple color fluorescence images.

  • Total 4.13 million pixels (1344 x 1024 x 3)
  • Total 36 bit color resolution
  • Cooling temperature of 0 °C
  • Individual R, G,and B exposure settings
  • 8x8 binning capability
  • Frame rate 9 Hz (49 Hz with 8x8 binning)
  • 12-bit readout; dynamic range 1380:1
  • Front or rear mount cable entry models (C7780-10 or C7780-20)

For further details of the ORCA-Flash2.8, ImagEM, ORCA-D2, and ORCA-R2, please see Hamamatsu's pages on Scientific Cameras.

For other cameras, please see the main Hamamatsu web site.

For some useful tutorials and articles on digital cameras - from basic to advanced - please see the Hamamatsu Learning Center

For more information and pricing details, please email us.


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