Bath Perfusion

Recording Chambers - closed and open baths for a large variety of experiments

Temperature Control - heated stages, heated chambers, inline solution heaters, controllers

Essential Supplies!

• Polyethylene Tubing - needed for any perfusion system. Common sizes are:

  • PE-160 Tubing - use for all Series 20 and RC chambers, as well as MP and MPP manifolds, and SH-27A & SH-27G Solution Heaters. OD 1.57 mm x ID 1.14 mm; available in 3 m and 30 m lengths.
  • PE-50 Tubing - for ML series manifolds and MM manifold inputs. OD 0.965 mm x ID 0.58 mm; available in 3 m lengths
  • PE-10 Tubing - for MM manifold outputs. OD 0.61 mm x ID 0.28 mm; available in 3 m lengths

• Coverslips - to suit all chambers; from 12 mm round to 24 x 60 mm rectangular.

• Silicone Grease - Dow Corning 111 Grease in 130 g tubes; maintains its viscosity even in heated chamber systems.

Perfusion Manifolds

Perfusion manifolds are useful wherever you need to connect multiple perfusion lines converging to a single output.

• MP Series Manifolds - designed for gravity perfusion systems. Available with 2, 3, 4, 5, 6 or 8 inputs; use PE-160 connection tubing.
• MPP Series Manifolds - designed for pressurised perfusion systems, and have 18 g stainless steel tubing for input & output ports. Available with 2, 3, 4, 5, 6, 8 or 24 inputs; use PE-160 connection tubing.
• MM and ML Series Manifolds - small diameter suitable for low flow rates. Available with 2, 4 or 6 inputs. The MM series uses PE-50 for input and PE-10 for output, the ML series uses PE-50 for both input and output.

Modular Syringe Holders

These modular syringe holders make it simple to set up whatever you need for perfusion reservoirs. The base holder clamps to a retort stand, and extra holders for the same or different size syringes can be snapped onto either side of the base holder

• Available for 5, 10, 20, 30, 60 and 140 ml syringes
• Made from Delrin and stainless steel
• Base holder clamps to any vertical stand of 9.5 to 16 mm diameter

Constant Flow Syringes

A simple and effective solution to the problem of varying flow rates as syringe reservoirs become depleted. These syringes maintain a constant gravity head, typically to within 1%.

• Available in 5, 10, 20, 30 and 60 ml sizes
• For a suitable holder, please see the Syringe Holders above

Warner Solution/Vacuum Control Valves

These valves, FR-50 and FR-55S, are used for fine control of solutions in a gravity fed perfusion system or vacuum used to evacuate a chamber. When used for chamber evacuation, the FR-50 provides the fine vacuum control critical to a properly operating system.

• Solution flow rate adjustable from 0 to 10 ml/min (at 30 cm solution head)
• Model FR-55S includes on-off switch for interrupting the flow without disturbing the flow rate

Perfusion Valve Control Systems

Two models of perfusion valve systems from Warner Instruments, giving uncomplicated & reliable switching of perfusion flow.

• Available in 2-, 4- or 6-channel (2- and 4- are expandable to 6-channel later)

• VC-6 Standard Valves are pinch valves (solution only contacts tubing) and are 3-way (allowing flow to waste with flow off)

• VC-6 Teflon Valves are two-way valves (on/off) useful where chemical resistance is needed

• VC-6M Mini Valves are compact 3-way valves on a Teflon manifold (ideal with SF-77B below)

• The VC-6 Controller switches up to 6 valves manually or by logic (TTL) input

Cell Micro Controls Flow Controller

The cFlow 8 Channel Flow controller differs from other perfusion controllers. It uses on optical drop sensor that fits around standard dripsets to detect the presence of a drop thereby enabling control of the flow rate. This sensing combined with the built in firmware allows different channels to be used for different purposes. For example you could have 2 channels for background solutions using the flow sensors to control the rate and the other 6 channels for a superfusion device where you only want to switch solutions.

• Front panel controls or RS232 control
• Single flow rate knob setting may be applied to any channel for consistency
• Ideal for both rate control and perfusion switching

Perfusion Fast Step

A simple and effective solution delivery device for use in patch clamping and electrophysiology. Solutions flow continuously through adjacent parallel delivery tubes and a stepper motor moves the tube set so that a different tube is directed at the preparation. This gives rapid solution changes without switching through intervening solutions.

• Supplied with 3-barrel square glass tubing: spacing is 0.7 mm and stepping between adjacent barrels is 20 msec.

• By using theta glass pulled to a 300 um tip, stepping times of 1 msec can be achieved.

NEW RV-8 Rotary Perfusion Valve

The new RV-8 from Warner Instruments is a motor-driven rotary valve head and controller that allows delivery of 1 of 8 gravity fed solutions. It is compact enough to allow close placement to the perfusion chamber, minimising deadspace.

• Switching time 150 msec for adjacent channels
• Internal deadspace only 10 µl
• Maximum flow rate 10 ml/min
• Please ask us for more details