email: Sydney, Australia Tel: +61-2-9882-2882

Electroporation Electrodes

BTX manufacture wide range of speciality electrodes for electroporation/electrofusion
in vivo, in ovo and in vitro (including petri dishes, microslides and well plates).

In vivo/in ovo Electrodes

  • 2-Needle Array Electrode - an in vivo style electrode specifically designed for muscle gene delivery; supplied sterile in six-packs; 5 mm or 100 mm spacing

  • Genetrodes - needle-style reusable electrodes that are used for a variety of applications; gold tips, available in straight or bent styles

  • Caliper Electrodes - reusable caliper style electrodes in two sizes

  • Tweezertrodes - reusable, tweezer-style in vivo electrodes for drug or gene delivery in animals. Standard 11.5 cm tweezer that has been modified with stainless steel circular or disk electrodes (7 or 100 mm dia)

  • Genepaddles - paddle-style reusable electrodes suitable for a variety of applications, available in two sizes

In vitro Electrodes

The Petri Pulser PP35-2P is a reusable electroporation applicator designed to fit into each single well of a 6-well plate or an individual 35mm diameter petri dish. The PP35-2P consists of an electrode assembly embedded in a polyurethane holder with high voltage electrode cables. It is designed for the electroporation of adherent cells in situ or as an alternative to cuvette electroporation for larger volumes or multiple cell samples.

Petri Dish Electrodes are designed to be used with a 100 mm petri dish which functions as the electroporation chamber. To perform electroporation or electrofusion, the samples are placed in the petri dish and the electrode assembly is lowered into the dish. The electrode assembly is available in 2 mm gap sizes. They contain parallel stainless steel electrodes, which produce homogeneous fields. The electrodes are designed to electroporate volumes ranging from 10 to 50 ml.

Cell Fusion & Nuclear Transfer Electrodes

BTX Microslides are used primarily with the ECM� 2001 Electro Cell Manipulator. The Microslide fits on the microscope allowing observation of the alignment and fusion process. The Microslide 450 is composed of a glass microslide and two strips of 0.5 mm stainless steel tubing which functions as electrodes to provide a divergent field. These wires are set apart at different gaps to provide various field strengths and volumes. The Microslides have been used for embryo manipulation and cell or protoplast fusion.

The BTX Flat Electrode Chamber can be used for both electroporation and electrofusion. They are made of two rectangular, parallel plates of high-grade stainless steel that is press-fitted into a polysulfone base. They generate fields depending on the orientation of the grooved electrodes. Orientating the grooved sides of each electrode so that they face one another generates a divergent field for use in electrofusion experiments. The flat sides of each electrode will generate a homogenous field when facing one another.

The BTX Meander Fusion Chamber is a microslide with a novel design specifically used for electro cell fusion. This chamber is constructed of a conductive metal alloy, which has been deposited in a finger-like projection array on a glass microscope slide. This method of manufacturing creates a highly precise working area. The configuration is designed to give direct viewing of a surface area. This can be used for viewing dimer formation during alignment while under a microscope. The gaps are set at 0.2mm.

Multi-well Coaxial Electrodes

Coaxial Electrodes are designed to be used for electroporation of cells in a 96-well plate. The electrode assembly is available as a Single-Coaxial Electrode or Multi-Coaxial Electrode (8-microwell channel electrode assembly). The Coaxial Electrodes are circular shape and produce divergent fields for electroporation of up to 0.3 ml. The Coaxial Electrodes can be used with all BTX electroporation systems.

For more specifications and electroporation protocols, please see the BTX website.

For further information and pricing details, please email us.

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