Reusable Equilibrium Dialysers

MicroEquilibrium Dialyzer, for volumes of 10 µl to 10 ml

 

MicroEquilibrium DialyzerTM The Micro-Equilibrium DIALYZER is a unique equilibrium dialysis chamber for small samples (25 to 500µl). Due to the small volume of the chamber, very small amounts of sample are required for protein binding assays. Two chambers of equivalent volume are joined together with a membrane between them, as shown. When dialysis is complete the chambers can be opened at each end to extract the sample for analysis. The entire system can also be placed in a thermostat for temperature-controlled dialysis.

The Micro-Equilibrium DIALYZER can also be used with three chambers instead of two. One of the main advantages of using this configuration is that the results can be obtained without waiting for equilibrium to be reached, thus reducing the assay time. This is achieved by placing the assay compound in the central chamber; the binding component in one of the terminal chambers and control buffer, containing neither component, in the remaining chamber. Comparing the concentration of the assay compound in the two terminal chambers will then yield information on the binding characteristics of the assay components.

Advantages

• Easy to use
• Leak-proof
• Reusable
• Available for a range of sample sizes
• Membranes available with MWCOs to suit almost any application
• Autoclaveable
• Low protein binding
• High sample recovery
• Made of Teflon - totally inert

Applications

• Protein binding assays
• Protein-drug binding assays
• Receptor binding assays
• Ligand binding assays
• Protein-protein interations
• Protein-DNA interactions

Ordering Information - MicroEquilibrium Dialyzer (for membrane selection see Membranes)

2-Chamber System
Chamber volume µl	25	50	100	250	500
Total volume µl	50	100	250	500	1000
Qty of 1	BS4 74-1606	BS4 74-1607	BS4 74-1608	BS4 74-1609	BS4 74-1610
Qty of 5	BS4 74-1600	BS4 74-1601	BS4 74-1602	BS4 74-1603	BS4 74-1604
Additional (add-on) Chambers for 3-chamber system
Chamber volume µl	25	50	100	250	500
Qty of 1	BS4 74-1619	BS4 74-1611	BS4 74-1612	BS4 74-1613	BS4 74-1614
Qty of 5	BS4 74-1620	BS4 74-1615	BS4 74-1616	BS4 74-1617	BS4 74-1618