SimplePCI
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SimplePCI is a high-performance, modular imaging software system, with full support for
digital cameras, automated microscopes and microscope add-ons such as filter wheels and stages.
SimplePCI Modules
SimplePCI core application Automated Image Capture (AIC) Image Processing and Analysis (IPA) Dynamic Intensity Analysis (DIA) Motion Tracking and Analysis (MTA) Deconvolution Nearest-neighbor / No-neighbor (DNN)
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The SimplePCI Core application allows sophisticated image enhancements, presentations and analysis
operations to be easily applied to single images or image sequences. Features include:
- Multi-threaded application benefits from Windows XP, 2000, NT
- Multiple processor support
- Comprehensive monochrome and color intensity threshold
- Advanced graphical tools for drawing individual or grouped shapes
- Customize measurements with built-in equation editor
- View data in 2D and 3D graphs, tables or spreadsheets
- Copy data to an internal spreadsheet editor
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- Access long image sequences with playback speed control
- Annotate movies with automatic update of field details
- Display image sequences as minimum or maximum projections
- Create an image montage from large image sequences
- Merge and replay multiple fluorescence images on brightfield images
- Reconstruct 3D images from serial sections
- Automatic registration for multiple color components
- Export and play AVI movies from an image sequence
- Load or save over 50 image formats including TIF and lossless JPEG
- Adjustable look-up tables for enhanced image display
- Plot an image as a 3D isometric plot of XY and intensity
- View image pixel values, histograms and color scattergrams
- Annotate text, arrows and calibration scalebars into images
- Merge multiple fluorescence images on brightfield images
- Copy, save or print full color or montage components
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Features for Fluorescence Microscopy
SimplePCI has many features specifically designed to make fluorescence work easy. These include
- Up to 3 wavelengths are automatically kept together as the components of an image, and support
is included for capture of up to 5 wavelengths.
- Hardware control for multiple wavelength capture is simple, flexible and intuitive.
- For dynamic studies, SimplePCI achieves the highest frame rate (with live on-screen display)
of any package we have seen, while providing real-time graphical analysis.
- Image sequences are automatically saved in a data file, and can be analysed for changing fluorescence
intensities or ratios with a few clicks (see the DIA module).
The SimplePCI Automated Image Capture module (AIC) enhances SimplePCI to provide
cost-effective image capture from a wide array of cameras.
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AIC-Standard provides:
- Support for a wide variety of video and digital cameras
- Auto-exposure, color balance and auto-focus controls
- Comprehensive support of motorized microscopes
- XYZ stages, shutters, filter wheels, tunable monochromators, liquid crystal tunable filters and emission image splitter
- Automated capture of XYZ and wavelength scans over time
- Simultaneous multi-site time-lapse acquisition
- Digital or analog auto-focus at specified time intervals
- TTL I/O signals for electrophysiology synchronization
- On-line intensity histograms and profiles
- Real-time noise averaging, shading and background correction
- Pixel calibrations stored for multiple objectives
- High speed streaming of images directly to memory or disk
The optional Experiment Planner (AIC-EP) provides:
- Multi-site, multi-level time-lapse acquisition
- Advanced technology incorporating intuitive visual interface
- Drag and drop interface
- High-level programming language without complex code
- Multiple device and operation expansion by demand
- Flexible stage device control
- Debugging and feedback mode
- Predefined template library supplied
- Structured image storage
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The Image Processing and Analysis module (IPA) encourages the development of icon driven work-files (macros) for multiple application solutions.
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- Integrates with AIC module for on-line capture and analysis
- Process images using Median, Smooth, Gradient, Laplacian, Sobel, Kirsch, Erode, Dilate and other kernel filters
- Threshold monochrome or color images to identify objects using RGB or HSL, with training regions
- Modify detected objects using Separate, Erode, Dilate, Skeletonize, Prune, plus Accept/Reject and Draw/Erase editor functions
- Qualify objects for measurement by size, shape, position, color and intensity combinations
- Select from over 150 field and object measurements
- Customize measurements using built-in equation editor
- Interactively display measured data with statistics, histograms, tables and spreadsheet displays
- Automatically run work-files on a stored data file of image sequences for post analysis
- Export data easily into spreadsheets and reports
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SimplePCI Dynamic Intensity Analysis (DIA) provides the ultimate in high speed processing.
Adding DIA to SimplePCI makes it possible to measure the intensity of cells over time, and then to view the
results on a time graph.
With DIA, full measuring capability is available for both on-line and off-line analysis.
Interactive user control throughout an experiment makes DIA the ideal software for live cell application solutions.
- Threshold or manually define regions of interest for
- intensity measurement
- Acquire, process and analyze data on-line throughout the
- experiment
- Plot individual, multiple or average measurements over time
- Customize measurements with built-in equation editor
- Apply background correction live or mathematically
- Process ratio image data for Ca++, pH ratio...
- Stream images directly to memory or disk
- Control capture of images with adjustable time delays
- Add event markers by key press or electronically
- Interact between measurement graphs and image sequences
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Motion Tracking and Analysis (MTA) expands SimplePCI to enable objects to be tracked across
multiple time separated fields.
Fields are first collected and analyzed for size (Area), position (Center of Gravity) and time (Field Time).
The MTA module then rapidly tracks objects and reports distance moved with straight and curve line velocity.
- Fast Object Tracking
- Track Multiple Objects
- Adjustable Parameters
- Object Velocity
- Replay Objects with Track
- View Individual Object Trajectory
- Extensive Statistics
- Straight and Curve Line Velocity
- Save Tracked Objects as AVI
- Export Data to Spreadsheet
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The SimplePCI Deconvolution module (DNN) supplements SimplePCI to remove out-of-focus haze from optical image sections. The module supports nearest neighbor no neighbor and 2D blind deconvolution, for rapid deconvolution of images and image sequences.
- Based on established algorithms from AutoQuant Imaging, Inc (now Media Cybernetics)
- Remove blur from images using fast algorithms
- Apply to individual images or image sequences automatically
- Deblur multi-wavelength images
- Easy access to deblur settings
- Automatic point spread generation
- Apply to full image or image region for fast parameter tuning
- Integrated for image capture and deconvolution
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For further details, please see the Hamamatsu SimplePCI web site.
For more information and pricing details, please email us.
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