Micropipette Making

Features of the P-97 include:

• Exceptional reproducibility from pull to pull
• Environmental chamber around the pipette tips
• Consistent and reliable electrodes with tip diameters less than 0.1 µm
• Two symmetrical pipettes with each pull
• Automatically looping cycles for patch pipettes
• Programmable air pressure
• Storage for 100 programs, each lockable and date stamped
• Ramp test quickly establishes heating with a new filament or glass size
• Pre-installed sample programs for intracellular & patch pipettes
• Self-contained air supply with filtration system and humidity control
• Please see the next page for filaments

Sutter P-2000 Micropipette Puller

This unique puller uses a CO₂ laser as the heating source, and hence is able to pull both ordinary glasses and pure quartz pipettes. Quartz has exceptional properties for many research applications: high strength, absence of metal ions, low electrical noise, & absence of fluorescence.

• Capable of pulling quartz, borosilicate and aluminosilicate glass
• Fully programmable including heating filament characteristics
• No filament to burn out
• Pulls electrodes with tip diameters < 0.03 µm
• Expected laser operating life > 10 years before factory refurbishment
• Storage for 100 programs, each lockable and date stamped
• Pre-installed sample programs for intracellular & patch pipettes
• Optionally configured for optical fibres and nanospray tips

Sutter P-30 Micropipette Puller

The P-30 vertical puller is designed for pulling basic micropipettes and patch pipettes, with tips down to 0.3 µm and moderate taper lengths (10-15 mm).

• Micrometer adjusted trip point
• Full three digit digital controls for accurate setting of heat and pull values
• Constant current power supplies for filament and pull solenoid
• Dual (manually) switched heat settings for patch pulling
• Enclosed front to reduce variability caused by draughts
• Two heating assemblies available; platinum/iridium (recommended) or Nichrome coil
• Rubber padded jaws to minimize breakage of capillary tubing
• Uses a minimum of bench space
• Please see the next page for filaments

Sutter BV-10 Beveller

The BV-10 offers precision beveling of micropipette tips between 0.1 and 50 µm. The unique abrasive plate drive system is vibration free for greater control of the beveling process. Beveling can be accomplished very rapidly to produce consistent tip diameters.

• Vibration free magnetically coupled beveling surface
• Abrasive surface optically flat to a half wave (250 nm)
• Finest abrasive surface commercially available
• Synchronous clock motor insures stable rotation rate
• 3 kg steel baseplate adds additional dampening
• Integrated halogen lamp
• Robust micromanipulator controlling bevel angle and advancement
• Optional 80X stereo microscope
• Optional Impedance meter for use during bevelling

Glassworx deFonbrune Microforge

The deFonbrune Microforge is used to quickly and accurately manufacture the various micro-instruments (needles, scalpels, pipettes, handles, hooks, etc.) necessary for optimal use with all types of micro-manipulators. The variety and complexity of tools that can be fabricated with the F-2000 is limited only by the skill and creativity of the user. From simple pipette polishing to complex tool production, this robust Microforge is ideal for the job.

• Available without optics - we can fit your choice
• Available with stereo zoom or compound microscope
• Available with colour video optics
• Extensive application notes available

Fine Point Patch Pipette Microforge

The GlasswoRx Fine Point Series Microforges are specifically designed to provide smooth polishing and precise bending of small glass pipette tips. A precision filament holder mounts on a LWD objective, giving a simple but effective microforge system using the microscope stage system to hold the pipette.

• Also available as Model 550, which pressurises the pipette during polishing. This is reported to usefully reshape the tip during polishing.