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Ultra-Micro and Micro TipColumnsTM are a simple and fast way to perform small
sample preparation based on the physical properties of the sample.
The columns consist of micro-pipette tips containing one of a wide range of available column
materials contained by frits and/or cap. The sample can be applied to the top of the column or suctioned
through the column using a micro-pipette. The sample is then purified according to size and
shape, chemical composition, charge or other physico-chemical properties by selecting the appropriate
column material.
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Applications
- Sample concentration
- Protein purification
- Peptide purification
- Salt removal
- Buffer exchange
- Radiolabel removal
- Detergent removal
- Dye-terminator removal
- Affinity purification
- Oligonucleotide purification
- DNA purification
- Carbohydrate purification
- Glycoprotein purification
- Glycopeptide purification
- Mass spectrometry (MALDI, ESI-MS)
- HPLC
- High performance capillary electrophoresis (HPCE)
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Advantages
- Fast, simple, easy to use - here's a diagram of the steps
- Unrivaled range of column materials available
- No special equipment - uses standard pipette
- Suitable for samples from 25 to 100 µl
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